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selector plasmid  (Addgene inc)


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    Structured Review

    Addgene inc selector plasmid
    Selector Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frame+selector/pCAS9-mCherry-Frame+%2B1+(Plasmid+%2366940)/pm41904124-296-6-8
    Average 93 stars, based on 23 article reviews
    selector plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: SMG1:SMG8:SMG9-complex integrity supports efficient execution of nonsense-mediated mRNA decay.
    Article Snippet: .. One day after seeding, 2000 ng universal donor (pCRISPaint-myc-PuroR, Addgene plasmid # 80961; pCRISPaint-TagGFP2-PuroR, Addgene plasmid # 80970; both vectors were a gift from Veit Hornung), 1000 ng frame selector (pCAS9-mCherry-Frame + 0/ + 1/ + 2, Addgene plasmid # 66 939/# 66 940/# 66 941; all three plasmids were a gift from Veit Hornung), and 1000 ng target selector (pX330-SMG8 delKID-R57; pX330SMG8-IDT-AA; pX330-SMG9-chop94) were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. 2 days after transfection the cells were transferred to 10 cm dishes and 4 –5 days after transfection cells were selected with 0.75 –1.0 μg/ml P r c [ p g ( p ( d o m N ( C G n a P A C s w a w S a p u f a ( P P ( p 4 G C G g l i U F F T c w p P s w ( i a p S D ow nloaded from https://academ ic.oup.com /nar/article/54/5/gkag193/8521937 by guest on 15 M arch 2026 uromycin (InvivoGen).

    Article Title: SMG1:SMG8:SMG9-complex integrity maintains robustness of nonsense-mediated mRNA decay
    Article Snippet: .. One day after seeding 2000 ng universal donor (pCRISPaint- myc-PuroR, Addgene plasmid # 80961; pCRISPaint- TagGFP2-PuroR, Addgene plasmid # 80970; both vectors were a gift from Veit Hornung), 1000 ng frame selector (pCAS9-mCherry-Frame +0/+1/+2, Addgene plasmid #66939/#66940/#66941; all three plasmids were a gift from Veit Hornung) and 1000 ng target selector (px330-SMG8 delKID-R57; px330-SMG8- IDT-AA; px330-SMG9-chop94) were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. 2 days after transfection the cells were transferred to 10 cm dishes and 4-5 days after transfection cells were selected with 0.75-1.0 μg/ml Puromycin (InvivoGen).

    Article Title: SMG1:SMG8:SMG9-complex integrity supports efficient execution of nonsense-mediated mRNA decay
    Article Snippet: .. One day after seeding, 2000 ng universal donor (pCRISPaint-myc-PuroR, Addgene plasmid # 80961; pCRISPaint-TagGFP2-PuroR, Addgene plasmid # 80970; both vectors were a gift from Veit Hornung), 1000 ng frame selector (pCAS9-mCherry-Frame + 0/+1/+2, Addgene plasmid # 66 939/# 66 940/# 66 941; all three plasmids were a gift from Veit Hornung), and 1000 ng target selector (pX330-SMG8 delKID-R57; pX330-SMG8-IDT-AA; pX330-SMG9-chop94) were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. 2 days after transfection the cells were transferred to 10 cm dishes and 4–5 days after transfection cells were selected with 0.75–1.0 μg/ml Puromycin (InvivoGen).

    Transfection:

    Article Title: SMG1:SMG8:SMG9-complex integrity supports efficient execution of nonsense-mediated mRNA decay.
    Article Snippet: .. One day after seeding, 2000 ng universal donor (pCRISPaint-myc-PuroR, Addgene plasmid # 80961; pCRISPaint-TagGFP2-PuroR, Addgene plasmid # 80970; both vectors were a gift from Veit Hornung), 1000 ng frame selector (pCAS9-mCherry-Frame + 0/ + 1/ + 2, Addgene plasmid # 66 939/# 66 940/# 66 941; all three plasmids were a gift from Veit Hornung), and 1000 ng target selector (pX330-SMG8 delKID-R57; pX330SMG8-IDT-AA; pX330-SMG9-chop94) were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. 2 days after transfection the cells were transferred to 10 cm dishes and 4 –5 days after transfection cells were selected with 0.75 –1.0 μg/ml P r c [ p g ( p ( d o m N ( C G n a P A C s w a w S a p u f a ( P P ( p 4 G C G g l i U F F T c w p P s w ( i a p S D ow nloaded from https://academ ic.oup.com /nar/article/54/5/gkag193/8521937 by guest on 15 M arch 2026 uromycin (InvivoGen).

    Article Title: SMG1:SMG8:SMG9-complex integrity maintains robustness of nonsense-mediated mRNA decay
    Article Snippet: .. One day after seeding 2000 ng universal donor (pCRISPaint- myc-PuroR, Addgene plasmid # 80961; pCRISPaint- TagGFP2-PuroR, Addgene plasmid # 80970; both vectors were a gift from Veit Hornung), 1000 ng frame selector (pCAS9-mCherry-Frame +0/+1/+2, Addgene plasmid #66939/#66940/#66941; all three plasmids were a gift from Veit Hornung) and 1000 ng target selector (px330-SMG8 delKID-R57; px330-SMG8- IDT-AA; px330-SMG9-chop94) were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. 2 days after transfection the cells were transferred to 10 cm dishes and 4-5 days after transfection cells were selected with 0.75-1.0 μg/ml Puromycin (InvivoGen).

    Article Title: SMG1:SMG8:SMG9-complex integrity supports efficient execution of nonsense-mediated mRNA decay
    Article Snippet: .. One day after seeding, 2000 ng universal donor (pCRISPaint-myc-PuroR, Addgene plasmid # 80961; pCRISPaint-TagGFP2-PuroR, Addgene plasmid # 80970; both vectors were a gift from Veit Hornung), 1000 ng frame selector (pCAS9-mCherry-Frame + 0/+1/+2, Addgene plasmid # 66 939/# 66 940/# 66 941; all three plasmids were a gift from Veit Hornung), and 1000 ng target selector (pX330-SMG8 delKID-R57; pX330-SMG8-IDT-AA; pX330-SMG9-chop94) were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. 2 days after transfection the cells were transferred to 10 cm dishes and 4–5 days after transfection cells were selected with 0.75–1.0 μg/ml Puromycin (InvivoGen).



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